Workflow4Metabolomics Object Identifier: W4M00005
Digital Object Identifier: 10.15454/1.4811287270056958E12
Creator of the history: Yann Guitton
Maintainer: Yann Guitton (yann.guitton at oniris-nantes.fr)
Creation|Updating date: 2016-12-01
Format: Workflow4Metabolomics Galaxy histories
Size: 327 Mo
Keywords: susscrofa, ractopamine, lcms, preprocessing, batch correction, OPLS-DA
|Study: Characterization of the serum physiological variations induced by ractopamine administration in pigs.|
Dataset: The dataset contains 164 mzXML files created from Q-Exactive (*.raw) files. 124 samples and 40 QCs injected in 4 batches. Design: 2 Classes (Treated, Controls) from serum collected at day 3, 9, 16, 18, 23, 29 with for each sample 2 extraction procedure (M and MW):
Workflow: The workflow consists of the following steps: preprocessing with XCMS and CAMERA, batch correction (loess), quality controls filters (RT and QCs CV<0.30) and multivariate statistics.
Comments: Please note that, the original publication of the study by Tao PENG et al was made with R and SIMCA and that the presented workflow reproduces the same workflow in W4M Galaxy environment.
Rights: Creative Commons
- Tao PENG, Anne-Lise ROYER, Yann GUITTON, Bruno LE BIZEC, Gaud DERVILLY-PINEL (2017) Serum-based metabolomics characterization of pigs treated with ractopamine.
Raw data repository:
Please find more referenced W4M histories here.